WA3 phosphate buffered saline ®
Original price was: $9.90.$7.00Current price is: $7.00.
WA3 phosphate buffered saline ® (PBS) is a sterile isotonic buffer solution widely used in biological research, peptide reconstitution, and pharmaceutical applications. It maintains pH stability and osmotic balance, making it essential for laboratory and clinical protocols.
Description
WA3 phosphate buffered saline ®
WA3 phosphate buffered saline ® (PBS) is a sterile isotonic buffer solution widely used in biological research, peptide reconstitution, and pharmaceutical applications. It maintains pH stability and osmotic balance, making it essential for laboratory and clinical protocols.
Benefits of WA3 Phosphate Buffered Saline®
Clinical & Research Benefits
- pH stabilization: Maintains physiological pH (~7.4) for biological samples.
- Isotonic balance: Prevents osmotic stress in cells and tissues.
- Peptide reconstitution: Provides a stable medium for mixing peptides and proteins.
- Cell culture support: Used for washing cells and maintaining sterility.
- Diagnostic assays: Common buffer in ELISA, immunohistochemistry, and Western blotting.
Lifestyle & Wellness Benefits
- Reliable preservation: Ensures peptide and biologic stability.
- Ease of use: Ready‑to‑use sterile solution.
- Cost efficiency: Reduces waste in multi‑dose protocols.
Dosage & Use
- Standard concentration: 0.01 M phosphate buffer, 0.137 M NaCl, 0.0027 M KCl.
- pH: ~7.4 (physiological).
- Used in peptide reconstitution, cell washing, and dilution protocols.
- Available in sterile liquid or powder form for laboratory preparation.
Side Effects & Precautions
- Injection site irritation: Rare, if used improperly.
- Not therapeutic: PBS is a buffer, not a drug.
- Contamination risk: Must remain sterile to avoid microbial growth.
Availability
- Pharmaceutical suppliers: Available in sterile vials and bottles.
- Research labs: Standard buffer for biological experiments.
- Online peptide supply: Sold for laboratory use worldwide.
- Global markets: Widely distributed in USA, EU, Asia, and Africa.
🧪 Expanded Comparison – WA3 Phosphate Buffered Saline® vs Other Buffers
Let’s take a deeper dive into how WA3 PBS® compares with other commonly used laboratory buffers and diluents.
📊 Detailed Comparison Table
| Feature | WA3 PBS® | Sterile Water | Tris Buffer | Bacteriostatic Saline |
|---|---|---|---|---|
| Type | Isotonic phosphate buffer | Pure diluent | Hydroxymethyl buffer | Saline with benzyl alcohol preservative |
| pH Control | Maintains physiological pH (~7.4) | No buffering capacity | Strong buffering (pH 7–9) | Mild buffering with NaCl |
| Osmotic Balance | Isotonic with cells/tissues | Hypotonic, can cause lysis | Not isotonic | Isotonic, prevents cell stress |
| Sterility | Sterile, ready‑to‑use | Sterile, but single‑use only | Sterile when prepared | Sterile with preservative for multi‑dose |
| Use Case | Peptide reconstitution, cell washing, diagnostic assays | Dilution, reconstitution without buffering | DNA/RNA work, molecular biology assays | Multi‑dose peptide storage, injectable vials |
| Unique Advantage | Combines physiological pH + isotonicity | Simple diluent, no additives | Stronger buffering for molecular biology | Preserves sterility in multi‑dose settings |
Expanded Benefits of WA3 PBS®
- Physiological compatibility: Mimics body fluid conditions, reducing stress on cells.
- Versatility in assays: Used in ELISA, immunohistochemistry, and Western blotting.
- Peptide stability: Provides a reliable medium for reconstitution.
- Ease of preparation: Available in sterile liquid or powder form.
- Global availability: Standard buffer in labs worldwide.
Dosage & Use
- Standard formula: 0.01 M phosphate buffer, 0.137 M NaCl, 0.0027 M KCl.
- pH: ~7.4, matching physiological conditions.
- Used for:
- Peptide reconstitution.
- Cell washing.
- Dilution protocols.
Side Effects & Precautions
- Injection site irritation: Rare, if used improperly.
- Not therapeutic: PBS is a buffer, not a drug.
- Contamination risk: Must remain sterile to avoid microbial growth.
Availability
- Pharmaceutical suppliers: Sterile vials and bottles.
- Research labs: Standard buffer for biological experiments.
- Online peptide supply: Sold globally for laboratory use.
- Clinical protocols: Used in diagnostic and therapeutic research.
The WA3 Phosphate Buffered Saline® solution stands out as a cornerstone buffer in both research and pharmaceutical applications. Unlike Sterile Water, which lacks buffering capacity, or Tris Buffer, which is strong but less physiological, WA3 PBS® offers the perfect balance of pH stability and isotonicity.
Its role in peptide reconstitution, diagnostic assays, and cell culture protocols makes it indispensable in modern laboratories. When compared to Bacteriostatic Saline, WA3 PBS® is more versatile for assays, while saline excels in multi‑dose preservation. Together, these buffers highlight different strategies for sterility, stability, and biological compatibility.
📌 Long Conclusion
The WA3 Phosphate Buffered Saline® solution is a cornerstone in biological research and peptide science. By maintaining pH stability and isotonic balance, it ensures the integrity of cells, peptides, and proteins during experiments.
Its versatility in peptide reconstitution, diagnostic assays, and cell culture protocols makes WA3 PBS® indispensable in both clinical and laboratory settings. With sterile preparation, global availability, and proven reliability, WA3 PBS® remains a trusted buffer solution for modern research and pharmaceutical applications.
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